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  • Jewgenow, K., Azevedo, A., Albrecht, M., Kirschbaum, C. & Dehnhard, M. Hair cortisol analyses in different mammal species: Choosing the wrong assay may lead to erroneous results. Conserv. Physiol. 8, 1–15 (2020).

    Article 

    Google Scholar
     

  • Saluti, G. et al. Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS. Anal. Bioanal. Chem. 414, 8093–8105 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Pyle Hennessey, E. M. et al. Hair cortisol and dehydroepiandrosterone concentrations: Associations with executive function in early childhood. Biol. Psychol. 155, 107946 (2020).

    Article 

    Google Scholar
     

  • Qiao, S. et al. Hair measurements of cortisol, DHEA, and DHEA to cortisol ratio as biomarkers of chronic stress among people living with HIV in China: Known-group validation. PLoS ONE 12, 1–15 (2017).

    Article 

    Google Scholar
     

  • de Graaff, A. M. et al. The associations of hair cortisol and DHEA with posttraumatic stress disorder symptoms in refugees. Compr. Psychiatry 129, 152438 (2024).

    Article 
    PubMed 

    Google Scholar
     

  • Pluchino, N. et al. Neurobiology of DHEA and effects on sexuality, mood and cognition. J. Steroid Biochem. Mol. Biol. 145, 273–280 (2015).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Probo, M. et al. Hair cortisol and dehydroepiandrosterone sulfate concentrations in healthy beef calves from birth to 6 months of age. Theriogenology 175, 89–94 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Bergamin, C. et al. Cortisol, DHEA, and sexual steroid concentrations in fattening pigs’ hair. Animals 9, 1–10 (2019).

    Article 

    Google Scholar
     

  • Olvera-maneu, S., Carbajal, A. & Gardela, J. Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes activity: Exploring the influence of seasonality. Stress 20, 1–10 (2021).


    Google Scholar
     

  • Nejad, J. G. et al. Effect of alpine grazing on plasma and hair cortisol, serotonin, and DHEA in dairy cows and its welfare impact. Domest. Anim. Endocrinol. 75, 106581 (2021).

    Article 
    CAS 

    Google Scholar
     

  • Peric, T. et al. Cortisol and DHEA concentrations in the hair of dairy cows managed indoor or on pasture. Livest. Sci. 202, 39–43 (2017).

    Article 

    Google Scholar
     

  • Arena, I. et al. Assessment of horses’ welfare: Behavioral, hormonal, and husbandry aspects. J. Vet. Behav. 41, 82–90 (2021).

    Article 

    Google Scholar
     

  • Weisser, J. J. et al. A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 1017–1018, 45–51 (2016).

    Article 

    Google Scholar
     

  • Hein, A. et al. Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry: Comparison with two immunoassays and application for longitudinal monitoring in zoos. Gen. Comp. Endocrinol. 310, 113837 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Sadok, I. et al. A validated LC–MS/MS method for simultaneous determination of key glucocorticoids in animal hair for applications in conservation biology. Sci. Rep. 13, 1–15 (2023).

    Article 

    Google Scholar
     

  • Gazzetta Ufficiale. Decreto Legislativo 4 marzo 2014, n. 26 (2014).

  • Sellick, J. Enhancing the protection of animals used for scientific purposes. Environ. Law Manag. 23, 75–82 (2011).


    Google Scholar
     

  • Duran, M. C., Janz, D. M., Waldner, C. L., Campbell, J. R. & Marques, F. J. Hair Cortisol concentration as a stress biomarker in horses: Associations with body location and surgical castration. J. Equine Vet. Sci. 55, 27–33 (2017).

    Article 

    Google Scholar
     

  • Schlink, A. C., Mata, G., Lea, J. M. & Ritchie, A. J. M. Seasonal variation in fibre diameter and length in wool of grazing Merino sheep with low or high staple strength. Aust. J. Exp. Agric. 39, 507–517 (1999).

    Article 

    Google Scholar
     

  • ICH. ICH guideline M10 on bioanalytical method validation. EMA Guid. Doc. 31, 1–57 (2022).

  • Cerasoli, F. et al. Assessment of welfare in groups of horses with different management, environments and activities by measuring cortisol in horsehair, using liquid chromatography coupled to hybrid orbitrap high-resolution mass spectrometry. Animals 12, 1–11 (2022).

    Article 

    Google Scholar
     

  • Higashi, T. & Ogawa, S. Chemical derivatization for enhancing sensitivity during LC/ESI–MS/MS quantification of steroids in biological samples: A review. J. Steroid Biochem. Mol. Biol. 162, 57–69 (2016).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Gao, W., Stalder, T. & Kirschbaum, C. Quantitative analysis of estradiol and six other steroid hormones in human saliva using a high throughput liquid chromatography-tandem mass spectrometry assay. Talanta 143, 353–358 (2015).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Stolze, B. R. et al. An improved micro-method for the measurement of steroid profiles by APPI-LC–MS/MS and its use in assessing diurnal effects on steroid concentrations and optimizing the diagnosis and treatment of adrenal insufficiency and CAH. J. Steroid Biochem. Mol. Biol. 162, 110–116 (2016).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Kalliokoski, O., Jellestad, F. K. & Murison, R. A systematic review of studies utilizing hair glucocorticoids as a measure of stress suggests the marker is more appropriate for quantifying short-term stressors. Sci. Rep. 9, 1–14 (2019).

    Article 
    CAS 

    Google Scholar
     

  • Leung, K. S. Y. & Fong, B. M. W. LC-MS/MS in the routine clinical laboratory: Has its time come?. Anal. Bioanal. Chem. 406, 2289–2301 (2014).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Delatour, T., Racault, L., Bessaire, T. & Desmarchelier, A. Screening of veterinary drug residues in food by LC-MS/MS. Background and challenges. Food Addit. Contam. Part A Chem. Anal. Control. Expo. Risk Assess. 35, 632–645 (2018).

    CAS 
    PubMed 

    Google Scholar
     

  • Matuszewski, B. K., Constanzer, M. L. & Chavez-Eng, C. M. Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS. Anal. Chem. 75, 3019–3030 (2003).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Thakare, R., Chhonker, Y. S., Gautam, N., Alamoudi, J. A. & Alnouti, Y. Quantitative analysis of endogenous compounds. J. Pharm. Biomed. Anal. 128, 426–437 (2016).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Binz, T. M., Braun, U., Baumgartner, M. R. & Kraemer, T. Development of an LC–MS/MS method for the determination of endogenous cortisol in hair using 13C3-labeled cortisol as surrogate analyte. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 1033–1034, 65–72 (2016).

    Article 

    Google Scholar
     

  • Placci, M. et al. Natural horse boarding vs traditional stable: A comparison of hormonal, hematological and immunological parameters. J. Appl. Anim. Welf. Sci. 23, 366–377 (2020).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Medill, S. A., Janz, D. M. & McLoughlin, P. D. Hair cortisol and testosterone concentrations in relation to maturity and breeding status of male feral horses. Animals 13, 2129 (2023).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Comin, A. et al. Hair cortisol level as a retrospective marker of hypothalamic-pituitary-adrenal axis activity in horse foals. Vet. J. 194, 131–132 (2012).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Montillo, M. et al. The Effect of temperature, rainfall, and light conditions on hair cortisol concentrations in newborn foals. J. Equine Vet. Sci. 34, 774–778 (2014).

    Article 

    Google Scholar
     

  • Prinsloo, M., Hynd, P., Franklin, S., Weaver, S. & van den Boom, R. Hair cortisol concentration is inversely related to the severity of equine squamous gastric disease. Vet. J. 249, 58–59 (2019).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Banse, H. E., Getachew, F., Levy, M. & Smits, J. Influence of season and pituitary pars intermedia dysfunction on hair cortisol concentration in horses. Domest. Anim. Endocrinol. 72, 106375 (2020).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Gardela, J. et al. Temporary relocation during rest periods: Relocation stress and other factors influence hair cortisol concentrations in horses. Animals 10, 642 (2020).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Sauveroche, M., Henriksson, J., Theodorsson, E., Svensson Holm, A. C. & Roth, L. S. V. Hair cortisol in horses (Equus caballus) in relation to management regimes, personality, and breed. J. Vet. Behav. 37, 1–7 (2020).

    Article 

    Google Scholar
     

  • Mazzola, S. M. et al. Do you think i am living well? A four-season hair cortisol analysis on leisure horses in different housing and management conditions. Animals 11, 2141 (2021).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Lelláková, M. et al. Cortisol concentration in horsehair and its relationship to body location, coat colour, and gender. J. Equine Vet. Sci. 115, 104010 (2022).

    Article 
    PubMed 

    Google Scholar
     

  • Lanci, A. et al. Hair cortisol and DHEA-S in foals and mares as a retrospective picture of feto-maternal relationship under physiological and pathological conditions. Animals 12, 1266 (2022).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Jolivald, A., Ijichi, C., Hall, C. & Yarnell, K. The mane factor: Compliance is associated with increased hair cortisol in the horse. Appl. Anim. Behav. Sci. 258, 105819 (2023).

    Article 

    Google Scholar
     

  • Stubsjøen, S. M. et al. Assessment of chronic stress in sheep (part I): The use of cortisol and cortisone in hair as non-invasive biological markers. Small Rumin. Res. 132, 25–31 (2015).

    Article 

    Google Scholar
     

  • Sawyer, G., Webster, D. & Narayan, E. Measuring wool cortisol and progesterone levels in breeding maiden Australian merino sheep (Ovis aries). PLoS ONE 14, 1–9 (2019).

    Article 

    Google Scholar
     

  • Nielsen, S. S. et al. Welfare of sheep and goats during killing for purposes other than slaughter. EFSA J. 22, 1–64 (2024).


    Google Scholar
     

  • Berthe, F. et al. The role of EFSA in assessing and promoting animal health and welfare. EFSA J. 10, 1–10 (2012).

    Article 

    Google Scholar
     

  • EU Commission. Farm to Fork Strategy. DG SANTE/Unit Food Inf. Compos. Food Waste 23 (2020).

  • Ghassemi Nejad, J. et al. Wool cortisol is a better indicator of stress than blood cortisol in ewes exposed to heat stress and water restriction. Animal 8, 128–132 (2014).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Salaberger, T. et al. Influence of external factors on hair cortisol concentrations. Gen. Comp. Endocrinol. 233, 73–78 (2016).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Dulude-deBroin, F., Côté, S. D., Whiteside, D. P. & Mastromonaco, G. F. Faecal metabolites and hair cortisol as biological markers of HPA-axis activity in the Rocky mountain goat. Gen. Comp. Endocrinol. 280, 147–157 (2019).

    Article 
    CAS 

    Google Scholar
     

  • Fürtbauer, I., Solman, C. & Fry, A. Sheep wool cortisol as a retrospective measure of long-term HPA axis activity and its links to body mass. Domest. Anim. Endocrinol. 68, 39–46 (2019).

    Article 
    PubMed 

    Google Scholar
     

  • Ghassemi Nejad, J. et al. Measuring hair and blood cortisol in sheep and dairy cattle using RIA and ELISA assay: A comparison. Biol. Rhythm Res. 51, 887–897 (2020).

    Article 
    CAS 

    Google Scholar
     

  • Weaver, S. J. et al. Chronic elevation of plasma cortisol causes differential expression of predominating glucocorticoid in plasma, saliva, fecal, and wool matrices in sheep. Domest. Anim. Endocrinol. 74, 106503 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Hantzopoulou, G. C., Sawyer, G., Tilbrook, A. & Narayan, E. Intra- and inter-sample variation in wool cortisol concentrations of Australian merino lambs between twice or single shorn ewes. Front. Anim. Sci. 3, 1–9 (2022).

    Article 

    Google Scholar
     

  • Zeinstra, E. C., Vernooij, J. C. M., Bentvelzen, M., van der Staay, F. J. & Nordquist, R. E. Wool cortisol as putative retrospective indicator of stress in ewes during the third trimester of pregnancy, and their newborns: Effects of parity and litter size—An exploratory study. Front. Anim. Sci. 4, 1–12 (2023).

    Article 

    Google Scholar
     

  • Oliveira, F. A. D. S., Pereira, E. N. C., Gobbi, J. M., Soto-Blanco, B. & Melo, M. M. Multiresidue method for detection of pesticides in beef meat using liquid chromatography coupled to mass spectrometry detection (LC-MS) after QuEChERS extraction. Food Addit. Contam. Part A Chem. Anal. Control. Expo. Risk. Assess. 35, 94–109 (2018).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Castellani, F., Ricci, M., Colagrande, M. N., Scortichini, G. & Saluti, G. Development and validation of a confirmatory method for the determination of 12 coccidiostat residues in eggs and muscle by means of liquid chromatography coupled to hybrid high resolution mass spectrometry. Separations 10, 202 (2023).

    Article 
    CAS 

    Google Scholar
     



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